Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions

Y Yang, P Babiak, JL Reymond

Index: Yang, Yongzheng; Babiak, Peter; Reymond, Jean-Louis Organic and Biomolecular Chemistry, 2006 , vol. 4, # 9 p. 1746 - 1754

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Citation Number: 33

Abstract

FRET-based fluorogenic substrates for lipases and esterases were prepared in four steps from commercially available building blocks. The substrates are pyrenebutyric acid monoesters of aliphatic 1, 2-diols bearing a dinitrophenylamino group as a quencher. The most enzyme-reactive substrate is ester 2a. The substrates do not show any measurable background reaction in the absence of enzyme even at pH 11, but react fast and ...