Microsphere??Based Real??Time Calcium Sensing

…, M Cuttle, S Mittoo, M Bradley

Index: Sanchez-Martin, Rosario M.; Cuttle, Matt; Mittoo, Stifun; Bradley, Mark Angewandte Chemie - International Edition, 2006 , vol. 45, # 33 p. 5472 - 5474

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Citation Number: 67

Abstract

Fluorescent sensors for a variety of ions, such as Ca2+ and Na+, are among the most useful tools available for studying ion fluxes within the intracellular environment following the modulation or stimulation of a range of complex biological processes. Two widely used fluorescent indicators for Ca2+ ions are Indo-1 (1) and Fura-2 (2), which complex calcium with high affinity (Kd= 230 and 145 nm, respectively), and these reagents have allowed ...