Vinyl sulfone bifunctional tag reagents for single-point modification of proteins

J Morales-Sanfrutos, FJ Lopez-Jaramillo…

Index: Morales-Sanfrutos, Julia; Lopez-Jaramillo, Francisco Javier; Hernandez-Mateo, Fernando; Santoyo-Gonzalez, Francisco Journal of Organic Chemistry, 2010 , vol. 75, # 12 p. 4039 - 4047

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Citation Number: 33

Abstract

The introduction of multiple labels onto biomolecules is a challenge. We report herein the synthesis of vinyl sulfone derivatized bifunctional tag single-attachment-point reagents (BTSAP) bearing biotin and a fluorescent tag and their applications in proteins for the introduction of multiple labels by means of the Michael-type addition of the electrophilic vinyl sulfone group. These BTSAP reagents were easily synthesized by a two-step chemical ...