Biochemistry

Binding of 2-naphthols to D38E mutants of 3-oxo-Δ5-steroid isomerase: Variation of ligand ionization state with the nature of the electrophilic component

IP Petrounia, G Blotny, RM Pollack

Index: Vesely; Jakes Bulletin de la Societe Chimique de France, 1923 , vol. <4> 33, p. 952

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Citation Number: 17

Abstract

... Volume: Page: ... Initial rates for D38E and D38E/D99A were fit to eq 4, whereas kinetic data for D38E/Y14F were fit to eq 5 (since the ... Dissociation constants for the binding of naphthols to the D38E mutants were determined by fluorescence quenching titration (20 ...