Enzymatic production of L-malate from maleate by Alcaligenes sp.

T Kimura, Y Kawabata, E Sato

Index: Kimura, Takuhei; Kawabata, Yasuro; Sato, Eiji Agricultural and Biological Chemistry, 1986 , vol. 50, # 1 p. 89 - 94

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Citation Number: 19

Abstract

Alcaligenes sp. T501, which has the ability to produce l-malate from maleate at a concentration of 20% in a molar yield of 98.4%, was isolated from soil. Analysis of the reaction mixture components suggested that maleate was converted to l-malate via fumarate through the action of maleate isomerase and fumarase. These two enzymes were constitutively found in the cells regardless of the carbon source in the medium. The ...