Characterization of chlorophenol 4-monooxygenase (TftD) and NADH: FAD oxidoreductase (TftC) of Burkholderia cepacia AC1100

BN Webb, JW Ballinger, E Kim, SM Belchik…

Index: Webb, Brian N.; Ballinger, Jordan W.; Kim, Eunjung; Belchik, Sara M.; Lam, Ka-Sum; Youn, Buhyun; Nissen, Mark S.; Xun, Luying; Kang, Chulhee Journal of Biological Chemistry, 2010 , vol. 285, # 3 p. 2014 - 2027

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Citation Number: 37

Abstract

Abstract Burkholderia cepacia AC1100 completely degrades 2, 4, 5-trichlorophenol, in which an FADH 2-dependent monooxygenase (TftD) and an NADH: FAD oxidoreductase (TftC) catalyze the initial steps. TftD oxidizes 2, 4, 5-trichlorophenol (2, 4, 5-TCP) to 2, 5- dichloro-p-benzoquinone, which is chemically reduced to 2, 5-dichloro-p-hydroquinone (2, 5- DiCHQ). Then, TftD oxidizes the latter to 5-chloro-2-hydroxy-p-benzoquinone. In those ...