Artificial metalloenzymes:(strept) avidin as host for enantioselective hydrogenation by achiral biotinylated rhodium-diphosphine complexes

…, N Humbert, J Collot, J Gradinaru, G Klein…

Index: Skander, Myriem; Humbert, Nicolas; Collot, Jerome; Gradinaru, Julieta; Klein, Gerard; Loosli, Andreas; Sauser, Jerome; Zocchi, Andrea; Gilardoni, Francois; Ward, Thomas R. Journal of the American Chemical Society, 2004 , vol. 126, # 44 p. 14411 - 14418

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Citation Number: 142

Abstract

We report on the generation of artificial metalloenzymes based on the noncovalent incorporation of biotinylated rhodium-diphosphine complexes in (strept) avidin as host proteins. A chemogenetic optimization procedure allows one to optimize the enantioselectivity for the reduction of acetamidoacrylic acid (up to 96% ee (R) in streptavidin S112G and up to 80% ee (S) in WT avidin). The association constant between a ...