Fluorometric determination of 1, 2, 3, 4-tetrahydro-6, 7-dihydroxyisoquinoline in biological materials by HPLC.

A Shirahata, M Yoshioka, Z Tamura

Index: Shirahata, Akira; Yoshioka, Masanori; Tamura, Zenzo Chemical and Pharmaceutical Bulletin, 1997 , vol. 45, # 11 p. 1814 - 1819

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Citation Number: 8

Abstract

In the belief that endogenous 1, 2, 3, 4-tetrahydro-6, 7-dihydroxyisoquinoline (DA-Fp) could be a potential marker involved in the etiology of various diseases such as Parkinsonism, we attempted to develop a fluorescence method for DA-Fp. It was synthesized by condensation of dopamine with formaldehyde according to an established method. Periodate was identified by screening from various oxidation reagents as a fluorescence reagent to DA- ...