3 S-Fluoroproline as a probe to monitor proline isomerization during protein folding by 19F-NMR
CA Thomas, ER Talaty, JG Bann
Index: Thomas, Colin A.; Talaty, Erach R.; Bann, James G. Chemical Communications, 2009 , # 23 p. 3366 - 3368
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Citation Number: 3
Abstract
The idea to monitor directly the population of proline cis–trans isomers in the unfolded state is not new, and was pioneered initially by Torchia and co-workers, through the biosynthetic incorporation of [4- 13 C] proline into collagen, and later applied to the study of staphylococcal nuclease. 5 Although methods and instrumentation (cryogenic probes) have been developed to improve sensitivity, carbon remains one of the least sensitive nuclei for detection and thus has ...
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