Controlled coupling of aminoglycoside antibiotics to proteins for use in homogeneous enzyme immunoassays

P Singh, M Pirio, DK Leung…

Index: Singh; Pirio; Leung; Tsay Canadian Journal of Chemistry, 1984 , vol. 62, # 11 p. 2471 - 2477

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Citation Number: 9

Abstract

Selective N-acylation of aminoglycoside antibiotics with the N-hydroxysuccinimide ester of methyldithioacetic acid, followed by reaction with methanethiol or dithioerythritol, gives sulfhydryl labeled antibiotics. Alternatively, the nucleophilic sulfhydryl group is incorporated into an antibiotic by treatment with N-acetyl-d, l-homocysteine thiolactone. These derivatives couple readily with proteins that have previously been modified with bromoacetylglycyl ...