Altering the specificity of subtilisin Bacillus lentus through the introduction of positive charge at single amino acid sites

BG Davis, K Khumtaveeporn, RR Bott…

Index: Davis, Benjamin G.; Khumtaveeporn, Kanjai; Bott, Richard R.; Jones, J.Bryan Bioorganic and Medicinal Chemistry, 1999 , vol. 7, # 11 p. 2303 - 2311

Full Text: HTML

Citation Number: 30

Abstract

The use of methanethiosulfonates as thiol-specific modifying reagents in the strategy of combined site-directed mutagenesis and chemical modification allows virtually unlimited opportunities for creating new protein surface environments. As a consequence of our interest in electrostatic manipulation as a means of tailoring enzyme activity and specificity, we have recently adopted this approach for the controlled incorporation of multiple ...