Development of an enzyme-linked immunosorbent assay for the detection of the organophosphorus insecticide acephate

…, DW Stoutamire, SJ Gee, BD Hammock

Index: Lee, Jae Koo; Ahn, Ki Chang; Stoutamire, Donald W.; Gee, Shirley J.; Hammock, Bruce D. Journal of Agricultural and Food Chemistry, 2003 , vol. 51, # 13 p. 3695 - 3703

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Citation Number: 51

Abstract

A competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the organophosphorus insecticide acephate, O, S-dimethyl acetylphosphoramidothioate, was developed using a polyclonal antibody. Five different haptens mimicking the analyte were synthesized and conjugated with the carrier proteins bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH) by the N-hydroxysuccinimide active ester and ...