Construction of an L-isoleucine overproducing strain of Escherichia coli K-12

K Hashiguchi, H Takesada, E Suzuki…

Index: Hashiguchi, Ken-Ichi; Takesada, Hiroko; Suzuki, Eiichiro; Matsui, Hiroshi Bioscience, Biotechnology and Biochemistry, 1999 , vol. 63, # 4 p. 672 - 679

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Citation Number: 30

Abstract

The genes for a threonine deaminase that is resistant to feedback inhibition by l-isoleucine and for an active acetohydroxyacid synthase II were introduced by a plasmid into a l- threonine-producing recombinant strain of Escherichia coli K-12. Analysis of culture broth of the strain using 13C nuclear magnetic resonance suggested that α, β-dihydroxy-β- methylvalerate (DHMV) and α-keto-β-methylvalerate (KMV), the third and the fourth ...