Click chemistry to construct fluorescent oligonucleotides for DNA sequencing

TS Seo, Z Li, H Ruparel, J Ju

Index: Seo, Tae Seok; Li, Zengmin; Ruparel, Hameer; Ju, Jingyue Journal of Organic Chemistry, 2003 , vol. 68, # 2 p. 609 - 612

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Citation Number: 275

Abstract

“Click chemistry” 1, 3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence ...