Model chemistry for a covalent mechanism of action of orotidine 5'-phosphate decarboxylase

RB Silverman, MP Groziak

Index: Silverman, Richard B.; Groziak, Michael P. Journal of the American Chemical Society, 1982 , vol. 104, # 23 p. 6434 - 6439

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Citation Number: 66

Abstract

Abstract: Orotidine 5'-phosphate decarboxylase (ODase) catalyzes the conversion of orotidylate to uridylate, the last step in the de novo biosynthesis of pyrimidine nucleotides. Model reactions are described that support a covalent catalytic mechanism for this enzyme in which, following protonation of the carboxyl group of orotidylic acid, an active-site nucleophile undergoes a Michael addition to the C-5 position. This covalent complex ...