Synthesis and nucleic acid binding studies of novel pyrrolidinyl PNA carrying an N-amino-N-methylglycine spacer

…, C Suparpprom, P Duanglaor, P Harnyuttanakorn…

Index: Vilaivan, Tirayut; Suparpprom, Chaturong; Duanglaor, Preeyanut; Harnyuttanakorn, Pongchai; Lowe, Gordon Tetrahedron Letters, 2003 , vol. 44, # 8 p. 1663 - 1666

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Citation Number: 9

Abstract

Two novel pyrrolidinyl peptide nucleic acids comprising alternating sequences of thymine- modified d-or l-proline and an N-amino-N-methylglycine spacer were synthesized using solid-phase methodology. UV and CD titrations together with a gel-binding shift assay revealed that neither of the homothymine PNA decamers bind to their complementary DNA or RNA. This was considered to be due to an unfavorable secondary structure which could ...