Elucidation of the Mechanism of Polysaccharide Cleavage by Chondroitin AC Lyase from Flavobacterium h eparinum

CS Rye, SG Withers

Index: Rye, Carl S.; Withers, Stephen G. Journal of the American Chemical Society, 2002 , vol. 124, # 33 p. 9756 - 9767

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Citation Number: 67

Abstract

Chondroitin AC lyase from Flavobacterium heparinum degrades chondroitin sulfate glycosaminoglycans via an elimination mechanism resulting in disaccharides or oligosaccharides with Δ4, 5-unsaturated uronic acid residues at their nonreducing end. Mechanistic details concerning the ordering of the bond-breaking and-forming steps of this enzymatic reaction are nonexistent, mainly due to the inhomogeneous nature of the ...