Redox-active metal-containing nucleotides: synthesis, tunability, and enzymatic incorporation into DNA

H Weizman, Y Tor

Index: Weizman, Haim; Tor, Yitzhak Journal of the American Chemical Society, 2002 , vol. 124, # 8 p. 1568 - 1569

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Citation Number: 72

Abstract

Novel redox-active DNA labeling tags with tunable electrochemical potentials are modularly synthesized using (a) bis-substituted Ru2+ or Os2+ precursors (R2bpy) 2ML2,(b) substituted 2, 4-pentanediones or hydroxamic acids bearing a functionalized linker, and (c) modified nucleotides. DNA polymerase efficiently incorporates the metal-containing nucleotide triphosphate into DNA oligonucleotides.