Extending the applicability of native chemical ligation

LE Canne, SJ Bark, SBH Kent

Index: Canne, Lynne E.; Bark, Steven J.; Kent, Stephen B. H. Journal of the American Chemical Society, 1996 , vol. 118, # 25 p. 5891 - 5896

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Citation Number: 241

Abstract

A more general approach to native (amide-forming) chemical ligation of unprotected peptide segments is described that extends the technique beyond the previously reported X-Cys ligation site to now include X-Gly and Gly-X ligation sites. A peptide,[peptide1] αCOSR, is reacted with a second peptide, HSCH2CH2 (O)-Nα [peptide2], under conditions promoting thioester exchange. The intermediate thioester-linked product rearranges to form a ...