Clip-Phen Conjugates for the Specific Cleavage of Nucleic Acids

A Laurent, M Wright, A Laayoun, B Meunier…

Index: Laurent, Alain; Wright, Michael; Laayoun, Ali; Meunier, Bernard; Tissot, Ludivine; Pitie, Marguerite Nucleosides, Nucleotides and Nucleic Acids, 2007 , vol. 26, # 8-9 p. 927 - 930

Full Text: HTML

Citation Number: 1

Abstract

The synthetic nuclease, Clip-Phen copper complex (1), promotes a very efficient oxidative cleavage of nucleic acids in the presence of a reductant. This molecule is a promising tool for nucleic acids based diagnostics where there is a need to fragment the long DNA, or RNA targets, prior to their amplification or hybridization on DNA arrays. However, even if Clip-Phen mediated oxidation occurs selectively at the desoxy or ribose moiety, the resulting cleavage is random. In ...