An efficient chemical and enzymic synthesis of nicotinamide adenine dinucleotide (NAD+)

…, MA Findeis, VM Rios-Mercadillo, J Auge…

Index: Walt, David R.; Findeis, Mark A.; Rios-Mercadillo, Victor M.; Auge, Jacques; Whitesides, George M. Journal of the American Chemical Society, 1984 , vol. 106, # 1 p. 234 - 239

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Citation Number: 41

Abstract

Abstract: This paper describes syntheses of NAD'(la) and NADP'(lb) that use traditional synthetic methodology in conjunction with enzyme-catalyzed reactions. The key intermediate, nicotinamide mononucleotide (0-NMN, 4), is prepared in two steps from ribose 5-phosphate (5). NMN is coupled with AMP through the pyrophosphorolysis of ATP (3) using the enzyme NAD pyrophosphorylase. The reaction sequence proceeds in 60% overall ...