Direct quantitative analysis of enzyme-catalyzed reactions by two-dimensional nuclear magnetic resonance spectroscopy: adenylate kinase and …

GL Mendz, G Robinson, PW Kuchel

Index: Mendz, George L.; Robinson, Gae; Kuchel, Philip W. Journal of the American Chemical Society, 1986 , vol. 108, # 1 p. 169 - 173

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Citation Number: 22

Abstract

Abstract: The back-transformation method for the analysis of two-dimensional nuclear magnetic resonance cross-relaxation and exchange data has been employed to obtain the rate constants of the phosphoglyceromutase and adenylate kinase catalyzed reactions in vitro. The results are in excellent agreement with those obtained by other methods. It is shown that a single two-dimensional experiment for each catalyzed reaction is sufficient to ...