Near-infrared heavy-atom-modified fluorescent dyes for base-calling in DNA-sequencing applications using temporal discrimination

…, CV Owens, SE Romero, E Waddell, SH Kahn…

Index: Flanagan Jr., James H.; Owens, Clyde V.; Romero, Sarah E.; Waddell, Emanuel; Kahn, Shaheer H.; Hammer, Robert P.; Soper, Steven A. Analytical Chemistry, 1998 , vol. 70, # 13 p. 2676 - 2684

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Citation Number: 82

Abstract

A series of near-IR fluorescent dyes were prepared which contained an intramolecular heavy atom for altering the fluorescence lifetimes to produce a set of probes appropriate for base-calling in a single-lane DNA sequencing format. The heavy-atom modification consisted of an intramolecular halogen situated on a remote section of the chromophore in order to minimize the perturbation on the lifetimes and fluorescence quantum yields. In ...