Detailed Reaction Mechanism of Macrophomate Synthase EXTRAORDINARY ENZYME CATALYZING FIVE-STEP TRANSFORMATION FROM 2-PYRONES TO …

K Watanabe, T Mie, A Ichihara, H Oikawa…

Index: Watanabe, Kenji; Mie, Takashi; Ichihara, Akitami; Oikawa, Hideaki; Honma, Mamoru Bioscience, Biotechnology and Biochemistry, 2000 , vol. 64, # 3 p. 530 - 538

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Citation Number: 82

Abstract

Abstract Macrophomate synthase from the fungusMacrophoma commelinae IFO 9570 is a Mg (II)-dependent dimeric enzyme that catalyzes an extraordinary, complex five-step chemical transformation from 2-pyrone and oxalacetate to benzoate involving decarboxylation, C–C bond formation, and dehydration. The catalytic mechanism of the whole pathway was investigated in three separate chemical steps. In the first ...