Chemistry of the bleomycin-induced alkali-labile DNA lesion

M Aso, M Kondo, H Suemune…

Index: Aso, Mariko; Kondo, Mitsumasa; Suemune, Hiroshi; Hecht, Sidney M. Journal of the American Chemical Society, 1999 , vol. 121, # 39 p. 9023 - 9033

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Citation Number: 38

Abstract

Treatment of B-form DNA with the antitumor antibiotic bleomycin in the presence of Fe2+ and O2 affords both DNA strand scission and the formation of alkali-labile lesions, the proportion of which is quite sensitive to the concentration of O2 present. The alkali-labile lesions can undergo fragmentation cleanly in the presence of n-butylamine to afford DNA fragments containing 5'-and 3'-phosphate termini at the site of the alkali-labile lesion. The ...