Inhibition of thiamin diphosphate dependent enzymes by 3-deazathiamin diphosphate

…, CP Melero, D Hawksley, FJ Leeper

Index: Mann, Stephane; Perez Melero, Concepcion; Hawksley, Dan; Leeper, Finian J Organic and biomolecular chemistry, 2004 , vol. 2, # 12 p. 1732 - 1741

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Citation Number: 29

Abstract

3-Deazathiamin diphosphate (deazaTPP) and a second thiamin diphosphate (TPP) analogue having a benzene ring in place of the thiazolium ring have been synthesised. These compounds are both extremely potent inhibitors of pyruvate decarboxylase from Zymomonas mobilis; binding is competitive with TPP and is essentially irreversible even though no covalent linkage is formed. DeazaTPP binds approximately seven-fold faster ...