Automated parallel synthesis of 5′-triphosphate oligonucleotides and preparation of chemically modified 5′-triphosphate small interfering RNA

…, JG Lackey, L Zhang, A Dell, K McRae, S Shaikh…

Index: Zlatev, Ivan; Lackey, Jeremy G.; Zhang, Ligang; Dell, Amy; McRae, Kathy; Shaikh, Sarfraz; Duncan, Richard G.; Rajeev, Kallanthottathil G.; Manoharan, Muthiah Bioorganic and Medicinal Chemistry, 2013 , vol. 21, # 3 p. 722 - 732

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Citation Number: 12

Abstract

A fully automated chemical method for the parallel and high-throughput solid-phase synthesis of 5′-triphosphate and 5′-diphosphate oligonucleotides is described. The desired full-length oligonucleotides were first constructed using standard automated DNA/RNA solid-phase synthesis procedures. Then, on the same column and instrument, efficient implementation of an uninterrupted sequential cycle afforded the corresponding ...