Nature of the intermediate between 4a-hydroperoxyflavin and 4a-hydroxyflavin in the hydroxylation reaction of p-hydroxybenzoate hydroxylase. Synthesis of 6- …

A Wessiak, TC Bruice

Index: Wessiak,A.; Bruice,T.C. Journal of the American Chemical Society, 1981 , vol. 103, p. 6996

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Citation Number: 13

Abstract

(14) The 5-nitrouracils 1IIa-c:" were hydrogenated in aqueous solution over Pd on charcoal. After addition of 6 M sulfuric acid to make the solutions about 1 M, the catalyst was filtered off, and an excess of sodium nitrite was added. After 1 h and partial neutralization, the diazouracils Va-c were extracted with chloroform and precipitated with diethyl ether. Pure com-pounds were obtained by column chromatography on silica gel with chloroform or ...