Carbonylbis (L-methionine p-nitrophenyl ester). New reagent for the reversible intramolecular crosslinking of insulin

WD Busse, FH Carpenter

Index: Busse,W.-D.; Carpenter,F.H. Journal of the American Chemical Society, 1974 , vol. 96, p. 5947 - 5949

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Citation Number: 15

Abstract

9, 1962 (1970). cyanogen bromide cleavage reaction12 for specific splitting of peptide chains at methionine residues. The absence of methionine in most species of insulin13 coupled with the stability of insulin to the conditions of the cyanogen bromide cleavageI4 were key factors in the development of the reversible cross-linking reagent.