Synthesis and Hybridization Properties of DNA–PNA Chimeras Carrying 5-Bromouracil and 5-Methylcytosine

E Ferrer, A Shevchenko, R Eritja

Index: Ferrer, Elisenda; Shevchenko, Anna; Eritja, Ramon Bioorganic and Medicinal Chemistry, 2000 , vol. 8, # 2 p. 291 - 297

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Citation Number: 26

Abstract

The preparation of 5-bromouracil and 5-methylcytosine peptide nucleic acid (PNA) monomers is described. These PNA monomers were used for the preparation of several DNA–PNA chimeras and their hybridization properties are described. The substitution of cytosine by 5-methylcytosine in DNA–PNA chimeras increased duplex stability while substitution of thymine by 5-bromouracil maintained it. Moreover, binding of DNA–PNA ...