Chemical inactivation of lipase in organic solvent: a lipase from Pseudomonas aeruginosa TE3285 is more like a typical serine enzyme in an organic solvent than in …

T Nakatani, J Hiratake, K Yoshikawa…

Index: Nakatani, Takuji; Hiratake, Jun; Yoshikawa, Kazuhiro; Nishioka, Takaaki; Oda, Jun'ichi Bioscience, Biotechnology, and Biochemistry, 1992 , vol. 56, # 7 p. 1118 - 1123

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Citation Number: 23

Abstract

A microbial lipase from Pseudomonas aeruginosa TE3285 was treated in anhydrous diisopropyl ether with three kinds of serine-reactive reagents, ethyl p-nitrophenyl methylphosphonate (ENMP), diisopropyl fluorophosphate (DFP), and phenylmethylsulfonyl fluoride (PMSF) to lose its catalytic activity for both transesterification in an organic solvent and ester hydrolysis in aqueous system. In contrast with the facile inactivation in an ...