A synthetic procedure for the preparation of oligonucleotides without using ammonia and its application for the synthesis of oligonucleotides containing 0-4-alkyl …
R Eritja, J Robles, A Aviñó, F Alberico, E Pedroso
Index: Eritja; Robles; Avino; Albericio; Pedroso Tetrahedron, 1992 , vol. 48, # 20 p. 4171 - 4182
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Citation Number: 31
Abstract
The preparation of 5′-O-dimethoxytrityl (DMT) and p-nitrophenylethyl (NPEOC, NPE) protected nucleosides linked to 4-(2-hydroxyethyl)-3-nitrobenzoic acid derivatives is described. These products attached to controlled-pore glass supports and together with DMT and NPE-protected nucleoside cyanoethyl phosphoramidites allows for the first time the preparation of short oligonucleotides containing the ammonia sensitive mutagenic ...
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