Hapten design and development of an ELISA (enzyme-linked immunosorbent assay) for the detection of the mercapturic acid conjugates of naphthalene

MP Marco, BD Hammock, MJ Kurth

Index: Marco, Maria-Pilar; Hammock, Bruce D.; Kurth, Mark J. Journal of Organic Chemistry, 1993 ,  vol. 58,  # 26  p. 7548 - 7556

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Citation Number: 48

Abstract

Pharmacol. Ther. 1989, 41,339). A glutathione-based detoxification pathway leads to the formation of the mercapturic acid conjugates [NaphMA 1,(R)-N-acetyl-S-[(lR*, 2R*)-1, 2- dihydo-l-hydroxy-2-naphthyllcysteine and (R)-N-acetyl-S-[(1R*, 2R*)-1, 2-dihydro-2-hydroxy- l-naphthyl] c~ teine] which are excreted in urine. Herein we report the development of an immunoassay for the specific detection of these urinary metabolites. This study confirms ...