Protein engineering by in vivo incorporation of non-natural amino acids: Control of incorporation of methionine analogues by methionyl-tRNA synthetase

KL Kiick, DA Tirrell

Index: Kiick, Kristi L.; Tirrell, David A. Tetrahedron, 2000 , vol. 56, # 48 p. 9487 - 9493

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Citation Number: 115

Abstract

The incorporation of non-natural amino acids is an important strategy for engineering novel chemical and physical properties into natural and artificial proteins. The incorporation of amino acids into proteins in vivo is controlled in large part by the aminoacyl-tRNA synthetases (AARS). We have measured kinetic constants for in vitro activation of a set of methionine analogues by methionyl-tRNA synthetase (MetRS) via the ATP–PPi exchange ...