Inverse phosphotriester DNA synthesis using photochemically-removable dimethoxybenzoin phosphate protecting groups

MC Pirrung, L Fallon, DC Lever…

Index: Pirrung, Michael C.; Fallon, Lara; Lever, David C.; Shuey, Steven W. Journal of Organic Chemistry, 1996 , vol. 61, # 6 p. 2129 - 2136

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Citation Number: 54

Abstract

A method has been developed to prepare short DNA sequences using light to deprotect a nucleoside 3'-phosphotriester, generating a phosphodiester useful for coupling with a free 5'- OH-nucleotide. The dimethoxybenzoin group is used as the photochemically-removable protecting group for the 3'-phosphate. Cyanoethyl is most effective as the second protecting group on the phosphodiester. Because the method is directed at the preparation and use ...