Mitochondrial thioredoxin-responding off–on fluorescent probe

…, JH Han, JH Lee, HG Choi, C Kang…

Index: Lee, Min Hee; Lee, Jae-Hong; Kim, Jong Seung; Han, Ji Hye; Kang, Chulhun; Choi, Hyo Gil Journal of the American Chemical Society, 2012 , vol. 134, # 41 p. 17314 - 17319,6

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Citation Number: 79

Abstract

We synthesized a new probe, Mito-Naph, to visualize mitochondrial thioredoxin (Trx) activity in cells. A fluorescence off–on change is induced by disulfide cleavage of the probe, resulting from a reaction with Trx and subsequent intramolecular cyclization by the released thiolate to give a fluorescent product. By measuring the fluorescence at 540 nm, Trx activity can be detected at nanomolar concentrations (down to 50 nM) well below its physiological ...