Nicotinamide??Dependent Ene Reductases as Alternative Biocatalysts for the Reduction of Activated Alkenes

K Durchschein, S Wallner, P Macheroux…

Index: Durchschein, Katharina; Wallner, Silvia; MacHeroux, Peter; Schwab, Wilfried; Winkler, Thorsten; Kreis, Wolfgang; Faber, Kurt European Journal of Organic Chemistry, 2012 , # 26 p. 4963 - 4968

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Citation Number: 16

Abstract

Abstract Four NAD (P) H-dependent non-flavin ene reductases have been investigated for their ability to reduce activated C= C bonds in an asymmetric fashion by using 20 structurally diverse substrates. In comparison with flavin-dependent Old Yellow Enzyme homologues, a higher degree of electronic activation was required, because the best activities were obtained with enals and nitroalkenes rather than enones and carboxylic esters. Although ...