Biomarkers 2015-02-01

Methy-sens Comet assay and DNMTs transcriptional analysis as a combined approach in epigenotoxicology.

Alessio Perotti, Valeria Rossi, Antonio Mutti, Annamaria Buschini

Index: Biomarkers 20(1) , 64-70, (2015)

Full Text: HTML

Abstract

Epigenotoxicology needs simple and fast tools to assess xenobiotic epigenetic load. This work proposes a comet assay modification designed to detect global methylation changes (Methy-sens Comet) through enzymatic digestion with two restriction enzymes (HpaII, MspI). In the methylation-sensitive protocol tested for repeatability on A549 cells, nickel chloride induced hypermethylation and decitabine-induced hypomethylation. A concomitant assessment of DNA methyltransferases (DNMTs) genes transcriptional levels has been performed, to implement a multifunctional approach to epigenotoxicology. Methy-sens Comet showed a general good repeatability and sensitivity to methylation changes while DNMTs transcriptional levels granted additional proof of xenobiotic-induced impairment of methylome maintenance.

Related Compounds

  • Ethidium bromide
  • L-Glutamine
  • Nickel Chloride

Related Articles:

L. monocytogenes in a cheese processing facility: Learning from contamination scenarios over three years of sampling.

2014-10-17

[Int. J. Food Microbiol. 189 , 98-105, (2014)]

Alpha-fetoprotein, identified as a novel marker for the antioxidant effect of placental extract, exhibits synergistic antioxidant activity in the presence of estradiol.

2014-01-01

[PLoS ONE 9(6) , e99421, (2014)]

Vegetables' juice influences polyol pathway by multiple mechanisms in favour of reducing development of oxidative stress and resultant diabetic complications.

2014-04-01

[Pharmacogn. Mag. 10(Suppl 2) , S383-91, (2014)]

Glucose recognition proteins for glucose sensing at physiological concentrations and temperatures.

2014-07-18

[ACS Chem. Biol. 9(7) , 1595-602, (2014)]

Reservoirs of listeria species in three environmental ecosystems.

2014-09-01

[Appl. Environ. Microbiol. 80(18) , 5583-92, (2014)]

More Articles...