Biological & Pharmaceutical Bulletin 2015-01-01

ISG15 regulates RANKL-induced osteoclastogenic differentiation of RAW264 cells.

Tomoharu Takeuchi, Genki Shimakawa, Mayumi Tamura, Hideyoshi Yokosawa, Yoichiro Arata

Index: Biol. Pharm. Bull. 38(3) , 482-6, (2015)

Full Text: HTML

Abstract

Interferon-stimulated gene 15 kDa (ISG15) is a protein upregulated by interferon-β that negatively regulates osteoclastogenesis. We investigated the role of ISG15 in receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenic differentiation of murine RAW264 cells. RANKL stimulation induced ISG15 expression in RAW264 cells at both the mRNA and protein levels. Overexpression of ISG15 in RAW264 cells resulted in suppression of cell fusion in RANKL-stimulated cells as well as the reduced expression of ATP6v0d2, a gene essential for cell fusion in osteoclastogenic differentiation. These results suggest that ISG15 suppresses RANKL-induced osteoclastogenesis, at least in part, through inhibition of ATP6v0d2 expression.

Related Compounds

  • Sodium acetate
  • Titanium tetrachlo...
  • Sodium TCA
  • TRAP-14 trifluor...
  • Triton X-100
  • sodium ascorbate
  • Sodium Tartrate

Related Articles:

Aptamer-based polyvalent ligands for regulated cell attachment on the hydrogel surface.

2015-04-13

[Biomacromolecules 16(4) , 1382-9, (2015)]

Bacteriophage PBC1 and its endolysin as an antimicrobial agent against Bacillus cereus.

2015-04-01

[Appl. Environ. Microbiol. 81(7) , 2274-83, (2015)]

H4 histamine receptors inhibit steroidogenesis and proliferation in Leydig cells.

2014-12-01

[J. Endocrinol. 223(3) , 241-53, (2014)]

Loading and release mechanism of red clover necrotic mosaic virus derived plant viral nanoparticles for drug delivery of doxorubicin.

2014-12-29

[Small 10(24) , 5126-36, (2014)]

Decreased lipogenesis in white adipose tissue contributes to the resistance to high fat diet-induced obesity in phosphatidylethanolamine N-methyltransferase-deficient mice.

2014-10-01

[Biochim. Biophys. Acta 1851(2) , 152-62, (2015)]

More Articles...