Biotechnology Progress 2006-01-01

Beta-galactosidase catalyzed selective galactosylation of aromatic compounds.

Nicolas Bridiau, Selma Taboubi, Nejib Marzouki, Marie Dominique Legoy, Thierry Maugard

Index: Biotechnol. Prog. 22(1) , 326-30, (2006)

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Abstract

A new approach to galacto-oligosaccharides and galacto-conjugates synthesis performed by the beta-galactosidase from Kluyveromyces lactis is reported. The enzymatic galactosylation of eight kinds of adsorbed aromatic primary alcohols, in particular the two drugs guaifenesin and chlorphenesin, gave the corresponding beta-D-galacto-pyranosides in yields ranging between approximately 10% and 96%. For the first time, we have showed that the adsorption of acceptor substrates onto solid supports such as silica gel influences the yield and the selectivity of galacto-conjugates synthesis. In particular, we observed that adsorption of acceptor favored the synthesis of digalactosylated compounds.


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