Bioanalysis 2013-03-01

Quantitative imaging of a therapeutic peptide in biological tissue sections by MALDI MS.

Nozomi Takai, Yukari Tanaka, Ayahisa Watanabe, Hideo Saji

Index: Bioanalysis 5(5) , 603-12, (2013)

Full Text: HTML

Abstract

Therapeutic peptides and proteins are being increasingly explored as potential therapeutic agents for molecular-targeted therapy, and the requirement for quantitative bioanalytical tools for such molecules has been discussed.The distribution of octreotide, a synthetic octapeptide analog of somatostatin, in the liver and kidney of mice administered with the analog was clearly visualized by MALDI-Imaging MS (IMS). The developed MALDI-IMS analytical method successfully quantified the amount of octreotide on tissue sections (accuracy was 76-127%) and the 2,5-dihydroxybenzoic acid-based normalization method was effective.The results of this study suggest that MALDI-IMS enables the quantification of an administered therapeutic peptide on biological tissue sections, as well as visualization of the in vivo distribution of the peptide.

Related Compounds

  • Gentisic acid
  • Octreotide acetate
  • Sodium gentisate

Related Articles:

Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase.

2015-01-01

[Nat. Commun. 6 , 5961, (2015)]

Antimicrobial activity of natural products from the flora of Northern Ontario, Canada.

2015-06-01

[Pharm. Biol. 53(6) , 800-6, (2015)]

Comparison of Mannose, Ethylene Glycol, and Methoxy-Terminated Diluents on Specificity and Selectivity of Electrochemical Peptide-Based Sensors.

2015-07-07

[Anal. Chem. 87 , 6966-73, (2015)]

Design and Evaluation of Tumor-Specific Dendrimer Epigenetic Therapeutics.

2015-06-01

[ChemistryOpen 4 , 335-41, (2015)]

Proteomics profiling of ethylene-induced tomato flower pedicel abscission.

2015-05-21

[J. Proteomics 121 , 67-87, (2015)]

More Articles...