Enzymatic Kinetic Resolution of Silybin Diastereoisomers⊥

…, R Gažák, P Marhol, D Biedermann…

Index: Monti, Daniela; Gazak, Radek; Marhol, Petr; Biedermann, David; Purchartova, Katerina; Fedrigo, Mirko; Riva, Sergio; Kren, Vladimir Journal of Natural Products, 2010 , vol. 73, # 4 p. 613 - 619

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Citation Number: 29

Abstract

In nature, the flavonolignan silybin (1) occurs as a mixture of two diastereomers, silybin A and silybin B, which in a number of biological assays exhibit different activities. A library of hydrolases (lipases, esterases, and proteases) was tested for separating the silybin A and B diastereomers by selective transesterification or by stereoselective alcoholysis of 23-O- acetylsilybin (2). Novozym 435 proved to be the most suitable enzyme for the preparative ...