Biosensors and Bioelectronics 2015-04-15

A sensitive sequential 'on/off' SERS assay for heparin with wider detection window and higher reliability based on the reversed surface charge changes of functionalized Au@Ag nanoparticles.

Yi Zeng, Jin-Ju Pei, Li-Hua Wang, Ai-Guo Shen, Ji-Ming Hu

Index: Biosens. Bioelectron. 66 , 55-61, (2014)

Full Text: HTML

Abstract

A sequential 'on/off' dual mode SERS assay platform for heparin with wider detection window and higher reliability is constructed based on electrostatic forces, in which the highly protonated chitosan encapsulated p-Mercaptobenzoic acid coated Au@Ag core-shell nanoparticles undergo sequential aggregation/segregation upon the additive of heparin with a limit of detection of 43.74ng/mL (5.69U/mL) and a continuous concentration range of 50-800ng/mL (6.5-104U/mL), which are lower in sensitivity and wider in detection window than the most reported assay for heparin. Remarkably, the latter declined window over a range of 350-800ng/mL in contrast, which has not reported before, is extremely important in reliable and practical assay of heparin. Copyright © 2014 Elsevier B.V. All rights reserved.

Related Compounds

  • sodiumborohydride
  • Ascorbic acid
  • acetic acid
  • Sodium citrate
  • 4-mercaptobenzoic...
  • Stanolone
  • acetic acid

Related Articles:

A new route for synthesis of silver:gold alloy nanoparticles loaded within phosphatidylcholine liposome structure as an effective antibacterial agent against Pseudomonas aeruginosa.

2015-03-01

[J. Liposome Res. 25(1) , 38-45, (2015)]

Development of Man-rGO for Targeted Eradication of Macrophage Ablation.

2015-09-08

[Mol. Pharm. 12 , 3226-36, (2015)]

Dual-Mode SERS-Fluorescence Immunoassay Using Graphene Quantum Dot Labeling on One-Dimensional Aligned Magnetoplasmonic Nanoparticles.

2015-06-10

[ACS Appl. Mater. Interfaces 7 , 12168-75, (2015)]

One Step Preparation of Reduced Graphene Oxide/Pd-Fe3 O4 @Polypyrrole Composites and Their Application in Catalysis.

2015-09-01

[Chem. Asian J. 10 , 1940-7, (2015)]

Surface modification-induced phase transformation of hexagonal close-packed gold square sheets.

2015-01-01

[Nat. Commun. 6 , 6571, (2015)]

More Articles...