Nature Communications 2015-01-01

Substrate stress relaxation regulates cell spreading.

Ovijit Chaudhuri, Luo Gu, Max Darnell, Darinka Klumpers, Sidi A Bencherif, James C Weaver, Nathaniel Huebsch, David J Mooney

Index: Nat. Commun. 6 , 6364, (2015)

Full Text: HTML

Abstract

Studies of cellular mechanotransduction have converged upon the idea that cells sense extracellular matrix (ECM) elasticity by gauging resistance to the traction forces they exert on the ECM. However, these studies typically utilize purely elastic materials as substrates, whereas physiological ECMs are viscoelastic, and exhibit stress relaxation, so that cellular traction forces exerted by cells remodel the ECM. Here we investigate the influence of ECM stress relaxation on cell behaviour through computational modelling and cellular experiments. Surprisingly, both our computational model and experiments find that spreading for cells cultured on soft substrates that exhibit stress relaxation is greater than cells spreading on elastic substrates of the same modulus, but similar to that of cells spreading on stiffer elastic substrates. These findings challenge the current view of how cells sense and respond to the ECM.

Related Compounds

  • Chloroform
  • Hydrochloric acid
  • sodium chloride
  • Glycine
  • Formaldehyde
  • dichloroethane
  • Adipohydrazide
  • glutaraldehyde
  • Sodium deoxycholat...
  • Calcium sulfate

Related Articles:

A survey of the interactome of Kaposi's sarcoma-associated herpesvirus ORF45 revealed its binding to viral ORF33 and cellular USP7, resulting in stabilization of ORF33 that is required for production of progeny viruses.

2015-05-01

[J. Virol. 89(9) , 4918-31, (2015)]

Aqueous-core PEG-coated PLA nanocapsules for an efficient entrapment of water soluble anticancer drugs and a smart therapeutic response.

2015-01-01

[Eur. J. Pharm. Biopharm. 89 , 30-9, (2015)]

SSX2 is a novel DNA-binding protein that antagonizes polycomb group body formation and gene repression.

2015-01-01

[Nucleic Acids Res. 42(18) , 11433-46, (2014)]

The dynamics of giant unilamellar vesicle oxidation probed by morphological transitions.

2014-10-01

[Biochim. Biophys. Acta 1838(10) , 2615-24, (2014)]

The dual FAAH/MAGL inhibitor JZL195 has enhanced effects on endocannabinoid transmission and motor behavior in rats as compared to those of the MAGL inhibitor JZL184.

2014-09-01

[Pharmacol. Biochem. Behav. 124 , 153-9, (2014)]

More Articles...