Thermally modified azocasein--a new insoluble substrate for the determination of proteolytic activity.
I Safarík
Index: Biotechnol. Appl. Biochem. 9 , 323-324, (1987)
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Abstract
An insoluble chromogenic substrate for the determination of proteolytic activity was prepared by heating azocasein in a thin layer at 200 degrees C for 4 h in a hot-air thermostat. The activity of an extracellular bacterial proteinase produced by Bacillus sp. was determined with this new substrate.
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