Biotechnology and Bioengineering 1998-06-20

Selective separation of trypsin from pancreatin using bioaffinity in reverse micellar system composed of a nonionic surfactant.

M Adachi, K Shibata, A Shioi, M Harada, S Katoh

Index: Biotechnol. Bioeng. 58(6) , 649-53, (1998)

Full Text: HTML

Abstract

Selective separation of trypsin from a mixture involving many kinds of contaminating proteins, i.e., pancreatin, was achieved using trypsin inhibitor immobilized in the reverse micelles, which were composed of a nonionic surfactant, tetra-oxyethylene monodecylether. To determine the efficient operations throughout the whole separation process we examined the operating conditions, which affect the immobilization efficiency of trypsin inhibitor and also the forward and backward extractions of trypsin. Fifty percent of the recovery of trypsin from pancreatin was realized with no loss of activity of the recovered trypsin.Copyright 1998 John Wiley & Sons, Inc.

Related Compounds

  • 3,6,9,12-Tetraoxat...

Related Articles:

Surfactants, aromatic and isoprenoid compounds, and fatty acid biosynthesis inhibitors suppress Staphylococcus aureus production of toxic shock syndrome toxin 1.

2009-05-01

[Antimicrob. Agents Chemother. 53 , 1898-906, (2009)]

Surfactant interference on lipase catalysed reactions in microemulsions.

1992-01-01

[J. Chem. Technol. Biotechnol. 54(3) , 277-82, (1992)]

Hydrogen-bond-directed giant unilamellar vesicles of guanosine derivative: preparation, properties, and fusion.

2011-07-19

[Langmuir 27(14) , 8653-8, (2011)]

Unbinding-binding transition induced by molecular snaps in model membranes.

2000-02-01

[Biophys. J. 78(2) , 857-65, (2000)]

Membrane hydration and structure on a subnanometer scale as seen by high resolution solid state nuclear magnetic resonance: POPC and POPC/C12EO4 model membranes.

1995-05-01

[Biophys. J. 68(5) , 1960-5, (1995)]

More Articles...