Chemical & Pharmaceutical Bulletin 2003-05-01

Fluorescence studies on the interactions of barbaloin with bovine serum albumin.

Jianniao Tian, Jiaqin Liu, Jiyou Zhang, Zhide Hu, Xinguo Chen

Index: Chem. Pharm. Bull. 51(5) , 579-82, (2003)

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Abstract

The fluorescence quenching reactions of barbaloin with bovine serum albumin (BSA) in pH 7.20 Tris-HCl buffer solution were studied. The quenching mechanism of BSA by barbaloin was interpreted using the Stern-Volmer (S-V) mechanism. The binding constant K values were 2.78 x 10(5) (293 K), 1.87 x 10(5) (310 K), 1.25 x 10(5) (318 K), and the number of binding sites (n) were 1.18, 1.14, and 1.09, respectively. In addition, the thermodynamic functions enthalpy (deltaH degrees ) and entropy (deltaS degrees ) for the reaction were also calculated according to Vant's Hoff equation were -23.7 kJ/mol and 23.6 J/mol, respectively. Plausible explanations of the quenching mechanism are discussed on the basis of a hydrophobic interaction between barbaloin and BSA.

Related Compounds

  • Aloin

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