Talanta 2015-09-01

A label-free impedimetric immunosensor for direct determination of the textile dye Disperse Orange 1.

Jing Yang, Carolina Gomes da Rocha, Shengfu Wang, Antonio Aparecido Pupim Ferreira, Hideko Yamanaka

Index: Talanta 142 , 183-9, (2015)

Full Text: HTML

Abstract

A strategy for a label-free impedimetric immunosensor is described for detection of the textile dye Disperse Orange 1 (DO1). The compounds 1,12-diaminododecane (DADD) and then 1,7-diaminoheptane (DAH) were firstly successively grafted onto a glassy carbon electrode (GCE) surface by electro-oxidation of one amino group, while the other terminal amino group was modified with the antibody anti-DO1. The construction process of the immunosensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and capacitance measurements. The electron transfer resistance (Rct) exhibited an effective response to the affinity between the immobilized antibody and the antigen in solution. The linear range for the target compound was from 5.0 nmol L(-1) to 0.5 μmol L(-1) (R=0.9980), and the limit of detection (LOD) was 7.56 nmol L(-1). The proposed impedimetric immunosensor has the advantages of simplicity, cost-effectiveness, and sensitivity.Copyright © 2015 Elsevier B.V. All rights reserved.

Related Compounds

  • Acetonitrile
  • 1,12-Diaminododeca...
  • heptan-1,7-diamin

Related Articles:

Neuroprotective effect of modified Chungsimyeolda-tang, a traditional Korean herbal formula, via autophagy induction in models of Parkinson's disease.

2015-01-15

[J. Ethnopharmacol. 159 , 93-101, (2014)]

Biomolecular imaging with a C60-SIMS/MALDI dual ion source hybrid mass spectrometer: instrumentation, matrix enhancement, and single cell analysis.

2014-11-01

[J. Am. Soc. Mass Spectrom. 25(11) , 1897-907, (2014)]

Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking.

2014-07-01

[Autophagy 10(7) , 1241-55, (2014)]

Diclofenac toxicity in human intestine ex vivo is not related to the formation of intestinal metabolites.

2015-01-01

[Arch. Toxicol. 89(1) , 107-19, (2015)]

Methionine oxidation accelerates the aggregation and enhances the neurotoxicity of the D178N variant of the human prion protein.

2014-12-01

[Biochim. Biophys. Acta 1842(12 Pt A) , 2345-56, (2014)]

More Articles...