Journal of enzyme inhibition 1995-01-01

Regulation of arylsulfate sulfotransferase from a human intestinal bacterium by nucleotides and magnesium ion.

L Konishi-Imamura, D H Kim, M Koizumi, K Kobashi

Index: J. Enzym. Inhib. 8(4) , 233-41, (1995)

Full Text: HTML

Abstract

Arylsulfate sulfotransferase (ASST) from a human intestinal bacterium stoichiometrically catalyzed the transfer of a sulfate group from phenylsulfate esters to phenolic compounds. Pentachlorophenol, one of the selective inhibitors of phenol sulfoconjugation in mammalian tissues, inhibited both phenol and tyramine sulfation by ASST. Nucleotide triphosphates such as ATP, GTP, UTP and CTP, and pyrophosphate inhibited the ASST activity, whereas Mg2+ and Mn2+ activated the enzyme and prevented its inhibition by ATP and pyrophosphate. Equimolar binding of [alpha-] and [gamma-32P]ATP to the enzyme showed that the enzyme protein was not phosphorylated, but bound ATP. These results suggest that nucleotide triphosphates and divalent cations are important modulators in the control of ASST activity.

Related Compounds

  • Potassium 4-nitrop...

Related Articles:

Relationship between substrate activity and pKa value of phenols on sulfotransferase from Eubacterium A-44.

1992-12-01

[Biochem. Int. 28(4) , 725-34, (1992)]

Sulfating-activity and stability of cDNA-expressed allozymes of human phenol sulfotransferase, ST1A3*1 ((213)Arg) and ST1A3*2 ((213)His), both of which exist in Japanese as well as Caucasians.

1999-08-01

[J. Biochem. 126(2) , 271-7, (1999)]

Kinetic studies on a novel sulfotransferase from Eubacterium A-44, a human intestinal bacterium.

1991-01-01

[J. Biochem. 109(1) , 45-8, (1991)]

para-Nitrophenyl sulfate activation of human sulfotransferase 1A1 is consistent with intercepting the E[middle dot]PAP complex and reformation of E[middle dot]PAPS.

2009-10-23

[J. Biol. Chem. 284(43) , 29357-64, (2009)]

Natural killer cell cytolytic granule-associated enzymes. I. Purification, characterization, and analysis of function of an enzyme with sulfatase activity.

1991-08-01

[J. Immunol. 147(3) , 950-8, (1991)]

More Articles...