Biochemical and Biophysical Research Communications 1992-04-30

A genetically engineered purpurin/retinol-binding protein hybrid that binds to transthyretin.

H Melhus, L Rask

Index: Biochem. Biophys. Res. Commun. 184(2) , 938-44, (1992)

Full Text: HTML

Abstract

A mini-gene encoding rat retinol-binding protein (RBP) and a cDNA encoding chicken purpurin were separately transfected into HeLa cells. In contrast to RBP, expressed purpurin did not bind to transthyretin (TTR). A purpurin/RBP hybrid protein was constructed by substituting the cDNA sequence encoding the N-terminal 29 amino acids of purpurin for the corresponding part of RBP. The expressed hybrid molecule bound to the TTR-Sepharose. These results demonstrate that purpurin does not bind to TTR, that a functional purpurin/RBP hybrid can be constructed, and that the N-terminal coil of RBP is not required for TTR binding.

Related Compounds

  • purpurin

Related Articles:

Structural and optical properties of Purpurin for dye-sensitized solar cells.

2015-10-05

[Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 149 , 997-1008, (2015)]

Protection against the bacterial mutagenicity of heterocyclic amines by purpurin, a natural anthraquinone pigment.

1999-08-18

[Mutat. Res. 444(2) , 451-61, (1999)]

Toxicity and tumorigenicity of purpurin, a natural hydroxanthraquinone in rats: induction of bladder neoplasms.

1996-04-19

[Cancer Lett. 102(1-2) , 193-8, (1996)]

Modified porphyrins, chlorins, phthalocyanines, and purpurins: second-generation photosensitizers for photodynamic therapy.

1989-04-01

[Semin. Hematol. 26(2) , 157-73, (1989)]

Biochemical differentiation of nascent neurite junctions: unilateral localization of adheron components.

1990-02-01

[Brain Res. Dev. Brain Res. 51(2) , 205-16, (1990)]

More Articles...